Interaction of pepstatin with pig pepsinogen

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The structure of pepstatin.

The UV spectrum of pepstatin shows only end absorption. Strong absorptions centered at 1630cm"1 and 1540cm"1 in the infrared spectrum suggest that pepstatin is a kind of peptide. Pepstatin shows a negative reaction for ninhydrin, but positive for RydonSmith reagent. Pepstatin has no free basic function. It gives mono-methyl ester by treatment of diazomethane. The di-acetyl derivative of the met...

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Pepstatin Inhibition of Human Renin

Pepstatin has been shown to be an extremely potent inhibitor of human renin (Ki = 1.3 X lo-lo M). Kinetic analyses by several techniques show reversible interaction of pepstatin with renin and classical noncofipetitive inhibition. The high affinity of pepstatin for renin allows kinetic determination of the molarity of the enzyme in partially purified preparations. This method has been validated...

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Lactoyl-pepstatin (an acylated tetrapeptide) is much more readily soluble in aqueous media than the more common isovaleryl- and acetyl-pepstatins (acylated pentapeptides). However, the K1 value for inhibition of cathepsin D by lactoyl-pepstatin at pH 3.5 is approx. 10(-7) M, some two to three orders of magnitude weaker than has been obtained previously for isovaleryl- or acetyl-pepstatins. One ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1976

ISSN: 0264-6021

DOI: 10.1042/bj1570499